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| RP-A00054-0.2 | |
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The LacZ β-Galactosidase (or beta-gal) can hydrolyze lactose to galactose plus glucose, transgalactosylate to form allolactose, and hydrolyze allolactose. It is best known for its reaction with X-gal (5-bromo-4-chloro-3-indoyl-β-d-galactopyranoside) for blue/white selection and commonly used in mammalian cells as a reporter to monitor transfection efficiency and promoter activity. |
| Form | Liquid |
| Concentration | 1mg/mL |
| Full mRNA length | 3339 nt |
| Full mRNA Molecular Weight | 1.08×10^6 Da |
| Storage buffer | 1mM Sodium citrate, pH 6.5 |
| Storage condition | Store at -20°C for short term (<3 months), store at -80°C for long term. |
| Appearance | Clear and free of foreign particles |
| RNA Length | Expected size band detected |
| RNA Content | Target ± 5% |
| Integrity | ≥ 75% |
| OD260/OD280 | 1.70 ~ 2.30 |
| Capping Efficiency | ≥ 90% |
| Endotoxin | < 10 EU/mg |
| pH | Target ± 0.5 |
| Transfect 0.1 µg of mRNA using 0.5 µL of Lipofectamine™ MessengerMAX™ Transfection Reagent (or equivalent) for one well of cells in a 96-well plate following manufacturer’s instructions. Perform Gal-Screen™ β-Galactosidase Reporter Gene Assay the next day following ThermoFisher Scientific’s assay protocol. |
Expression of Beta-gal mRNA in A549 cells. X4 and X5 represent GenScript mRNA codon optimized using different algorithms; ComT represents competitor T. »
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
