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| M00648 | |
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| Product Description | Recombinant CHO-K1 cells stably overexpress Macaca fascicularis TNF receptor superfamily member 14 (HVEM) on the surface. The surface expression of HVEM is validated by FACS analysis. This cell line is recommended for cell-based binding assay to screen antibodies against cyno HVEM or to measure binding affinity between HVEM and anti- HVEM antibodies. |
| Culture Properties | Adherent |
| Stability | Stable through more than 15 passages without significant changes in assay performance or expression profile. |
| Size | Two vials of frozen cells (>1×106 per vial in 1 mL). |
| Storage | Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received. |
| Culture Medium | Ham’s F-12K (Kaighn’s), 10% FBS, 8 μg/ml Puromycin (Cat. No. A11138-03, Life Technologies) |
| Complete Growth Medium | Ham’s F-12K (Kaighn’s) (Cat. No. 21127-022, Life Technologies), 10% FBS (Cat. No. 10099-141, Life Technologies) |
| Freeze Medium-DATA | 95% complete growth medium, 5% (V/V) DMSO (Cat. No. D2650, Sigma) |
Figure 1. FACS analysis of cell surface expression of cyno HVEM on CHO-K1/cyno HVEM cells. The CHO-K1/cyno HVEM cells (blue) and the negative control CHO-K1 cells (red) were probed using PE-conjugated anti-HVEM antibody (Cat. No. 318806, Biolegend).
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
