| ￥2000 在线订购包邮(港澳台除外) | |
| RP-A00081-0.05 | |
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| 咨询产品问题 | |
The scarless CD19 CAR circular RNA (CVB3) encodes a chimeric antigen receptor (CAR) specific for CD19. This circRNA features a scarless circularization design, eliminating linker or junction sequences between the 5′ and 3′ ends, and enables efficient circularization and expression without the need for optimized UTR elements.
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| Form | Liquid |
| Concentration | 1mg/mL |
| Full mRNA Molecular Weight | 730168 Da |
| Full circRNA length | 2265 nt |
| Storage buffer | 1mM Sodium citrate, pH 6.5 |
| Storage condition | Store at -20°C for short term (<3 months), store at -80°C for long term. |
| Appearance | Clear and free of foreign particles |
| RNA Length | Expected size band detected |
| RNA Content | Target ± 5% |
| OD260/OD280 | 1.70 ~ 2.30 |
| Endotoxin | < 10 EU/mg |
| pH | Target ± 0.5 |
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NFAT Activation Assay (Luciferase) – CD19 CAR mRNA: In this assay, Jurkat cells were transfected with scarless CD19 CAR circular RNA (CVB3) and subsequently co-incubated with NLAM cells expressing the CD19 antigen to assess CAR-mediated signaling. Activation of the CAR triggered the NFAT signaling pathway, leading to luciferase expression under NFAT-responsive promoter control. The resulting luminescence intensity served as a quantitative measure of NFAT activation — where higher luminescence indicates stronger CAR activation and higher binding affinity between the CD19 CAR and its antigen. This assay provides a functional readout of CAR engagement and signaling potency. |
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For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
