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PiggyBac is a DNA transposon and a promising alternative to Sleeping Beauty. PiggyBac is originally isolated from the cabbage looper moth Trichoplusia ni genome, is distinguished by its ability to excise precisely. (Zheng Zhang, et al, J Comput Biol 2000; 7(1-2):203-14.) |
| Form | Liquid |
| Concentration | 1mg/mL |
| Full mRNA length | 2156 nt |
| Full mRNA Molecular Weight | 695831 Da |
| Storage buffer | 1mM Sodium citrate, pH 6.5 |
| Storage condition | Store at -20°C for short term (<3 months), store at -80°C for long term. |
| Appearance | Clear and free of foreign particles |
| RNA Length | Expected size band detected |
| RNA Content | Target ± 5% |
| Integrity | ≥ 75% |
| OD260/OD280 | 1.70 ~ 2.30 |
| Capping Efficiency | ≥ 90% |
| Endotoxin | < 10 EU/mg |
| pH | Target ± 0.5 |
| Transfect 200ng of PiggyBac mRNA and 800ng of donor DNA with eGFP sequence into 5×104 HEK293T cells using Lipofectamine™ 2000 (or equivalent). On day 3, 6, 10 and 12, use flow cytometry analysis to evaluate the ratio of eGFP positive cells and eGFP expression intensity. |
Editing performance of PiggyBac system in HEK293T cells. GC represents GenCircle, the donor DNA in GenCircle format with eGFP sequence. »
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
