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GenLNP-S01-EGFP mRNA (5MOU)

The LNP formulation loaded with eGFP mRNA (5MOU) is prepared using generic LNP formulation with main ionizable lipid of SM102. The payload EGFP mRNA (5MOU) has all the U bases 100% modified with 5-methoxy Uridine. It expresses an enhanced green fluorescent protein (Ex: 488nm, Em: 507nm). This formulation is a good control for testing if SM102 LNP is a good potential formulation for your mRNA product under research in your in vitro and in vivo experiment model.
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 RP-A00007-50

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Description

The LNP formulation loaded with eGFP mRNA (5MOU) is prepared using generic LNP formulation with main ionizable lipid of SM102.  
 
The payload EGFP mRNA (5MOU) has all the U bases 100% modified with 5’-Methoxyuridine (5-MOU). It expresses an enhanced green fluorescent protein, with the open reading frame sequence originally from the jellyfish, Aequorea Victoria. EGFP mRNA is a commonly used fluorescence reporter sequence in mRNA therapeutics and delivery system development projects. The expressed protein has an excitation wavelength of 488nm, emission at 507nm.  
 
 This formulation is a good control for testing if SM102 LNP formulation is a good potential formulation for your mRNA product under research in your in vitro and in vivo experiment model.
Form Liquid
Concentration 0.15mg/mL
Full mRNA length 980nt
Full mRNA Molecular Weight 319792
Storage buffer Tris-HCl/Sucrose
Storage condition Store at -80°C for long term. Avoid freeze thaw, only thaw once before use.
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Appearance Clear and free of foreign particles
Encapsulation Efficiency > 85%
Endotoxin < 4 EU/ml
Particle Size 65-125 nm
pH 7.4 ± 0.5​
Polydispersity Index < 0.2
Zeta Potential ± 15.0 mV
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1. LNP can be added directly to cells incubated in complete media 
2. Cells grown and transfected in complete media (DMEM +10% FBS) has higher expression than reduced serum medium 
3. For suspension cell lines, we recommend using low fluorescent background media such as Flourobrite® with 10% FBS, 1X GlutaMax® and 1X sodium pyruvate  
4. For adherent cell line, we recommend replace complete media with Opti-mem right before reading the cell eGFP expression 
5. For higher expression, we recommend incubate the LNP with FBS at 1:2 volume ratio (LNP:FBS) for 30 minutes at 37C before transfecting
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GenLNP-S01-EGFP MRNA (5MOU)

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 For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.