黄金城集团

目录产品 » 抗体产品 » 蛋白分析试剂 » Biotin-Protein L

Biotin-Protein L

Protein L is a cell surface protein from Peptostreptoccocus magnus that binds to the variable light chains (kappa chain) of immunoglobulins without interfering with antigen binding. In contrast to IgG-binding proteins, such as protein A and protein G, protein L can be used for the detection and purification of mammalian kappa light chain antibodies of all classes. Biotin-Protein L, which is produced by covalently linking biotin to protein L, can be used in immunodetections, such as immunoblotting (western or dot blot), ELISA, and immunohistochemistry.
￥980
M00097

Reconstitution Reconstitute the lyophilized product with deionized water (or equivalent) to the final concentration of 1.0 mg/ml.
Concentration 1 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide.
Storage The product remains stable in lyophilized form for 1 year if stored at 2-8°C, -20°C, or lower from the date of receipt. For longer storage, -20°C or below is recommended. The reconstituted product can be stored for 2-3 weeks at 2-8°C or for 1 year at -20°C or below. Avoid repeated freezing and thawing cycles.
返回

Working concentrations for specific applications should be determined by titration assay. The chart below may serve as a useful guideline, but primary antibody, temperature, incubation time, the amount of antigen, and the sensitivity of the detection methods can all affect the ideal working concentration.
Application Recommended Usage
Dot blot 1:2,000-1:20,000
Western blot 1:2,000-1:20,000
ELISA 1:20,000-1:100,000
Other applications user-optimized
返回

Biotin-Protein L

»

<
>

Target Background Protein L is a cell surface protein from Peptostreptoccocus magnus that binds to the variable light chains (kappa chain) of immunoglobulins without interfering with antigen binding. In contrast to IgG-binding proteins, such as protein A and protein G, protein L can be used for the detection and purification of mammalian kappa light chain antibodies of all classes. Biotin-Protein L, which is produced by covalently linking biotin to protein L, can be used in immunodetections, such as immunoblotting (western or dot blot), ELISA, and immunohistochemistry.
返回

 For research use only. Not intended for human and animal therapeutic or diagnostic use.


Xianjun Li, et al. Engineered outer membrane vesicles enhance solid tumour CAR-T cell therapy. Nature biomedical engineering. (2026-01)
Edward Z Song, et al. Engineered CXCR3-A expression enhances B7-H3-targeting CAR T cell migration and efficacy against diffuse intrinsic pontine glioma. Nature Communications. (2025-11)
Shin-Ichiro Takayanagi, et al. A culture method with berbamine, a plant alkaloid, enhances CAR-T cell efficacy through modulating cellular metabolism. Commun Biol. (2024-06)
Gerard J Chu, et al. The 4-1BBζ costimulatory domain in chimeric antigen receptors enhances CD8+ T-cell functionality following T-cell receptor stimulation. Cancer Cell Int. (2023-12)
Carbonneau S, et al. An IMiD-inducible degron provides reversible regulation for chimeric antigen receptor expression and activity. Cell chemical biology. (2020-12)
Cui J, et al. Inhibition of PP2A with LB-100 Enhances Efficacy of CAR-T Cell Therapy Against Glioblastoma. Cancers (Basel). (2020)
Yu Ping, et al. Augmenting the Effectiveness of CAR-T Cells by Enhanced Self-Delivery of PD-1-Neutralizing scFv. Front Cell Dev Biol. (2020)
Teilo H Schaller, et al. First in human dose calculation of a single-chain bispecific antibody targeting glioma using the MABEL approach. J Immunother Cancer. (2020)
Daria A. Matvienko , et al. Analysis of in vitro activity of PSCA-specific CARs in the context of human NK cell line YT. Cellular Therapy and Transplantation (CTT). . (2018-07)
Daria A, et al. Analysis of in vitro activity of PSCA-specific CARs in the context of human NK cell line YT. Cellular Therapy and Transplantation. (2018-07)
Andrijauskaite K, et al. IL-12 conditioning improves retrovirally mediated transduction efficiency of CD8+ T cells. Cancer Gene Ther. (2015-07)
查看更多
返回