黄金城集团

目录产品 » ELISA检测试剂盒 » cAMP ELISA Detection Kit

cAMP ELISA Detection Kit

GenScript cAMP ELISA Detection Kit is a competition enzyme-linked immunoassay which can be used for quantitative detection of cAMP in samples such as serum, plasma, saliva, cell culture supernatant, and urine.
￥2870 在线订购包邮(港澳台除外)
L00460

咨询产品问题
Product Description Adenosine 3’, 5’ cyclic monophosphate (cAMP) is an important secondary messenger in signal transduction pathways that follows a number of extracellular signals. The G-Protein Coupled Receptors (GPCRs) are critical cell surface receptors that can be activated by different ligands, such as neurotransmitters, hormones and ions. The binding of the ligand to its receptor activates the effector adenylyl cyclase generating cAMP product. cAMP activates or inhibits various enzymes by promoting their phosphorylation or dephosphorylation. Its concentration is converted from adenosine triphosphate (ATP) via adenylyl cyclases (AC), and is inactivated by hydrolysis to 5´-AMP by the actions of phosphodiesterases.
GenScript cAMP ELISA Detection Kit is a competition enzyme-linked immunoassay which can be used for quantitative detection of cAMP in samples such as serum, plasma, saliva, cell culture supernatant, and urine. The anti-IgG Capture Plate is pre-coated with a fixed amount of Goat anti-mouse IgG to capture Mouse Anti-cAMP Monoclonal Antibody. When free cAMP or specimen and HRP-cAMP are added to the well, they compete in the solution to interact with the cAMP antibody captured on the plate. Other unbound molecules are removed by the wash step. The cAMP-HRP reacts with TMB Solution to develop a blue product in the solution. The reaction is stopped by adding stop solution and the color turns yellow which can be read at 450 nm by a Microtiter plate reader. Because the concentration of cAMP-HRP is held constant in the assay, the intensity of color is inversely proportional to the cAMP concentration in the sample and standards. Using the standard curve, the cAMP amount present in the unknown samples can be calculated by transforming its absorbance value.
Detection Range 1~243 pmol/ml
Sensitivity 0.61 pmol/ml
Test Samples Serum, plasma, saliva, cell culture supernatant, and urine
Conveniency All reagents and buffers for cAMP test are provided
Complete the test within 3.5 hours
Storage The unopened kit is stable for 12 months if stored at 2-8 °C, and the opened kit is stable for 2 weeks at 2-8 °C. Do not freeze the kit.
Kit Contents
Components Quantity Part.No
Anti-IgG Capture Plate 1 plate (8 wells x 12 strips) 460-80
Anti-cAMP mAb 12 ml 460-20
HRP-cAMP 6 ml 460-30
cAMP Standards (0, 1, 3, 9, 27, 81, 243 pmol/ml) 1.5 ml 460-11, 12, 13, 14, 15, 16,17
cAMP Standard Stock (10 nmol/ml) 500 μl 460-10
Assay Buffer A 60 ml 460-60
Assay Buffer B 1 ml 460-90
20× Wash Solution 40 ml B-A1-70
TMB Solution 12 ml B-A1-40
Stop Solution 6 ml B-A1-50
Plate Sealer 2 pieces N/A
返回

CAMP ELISA Detection Kit

»

CAMP ELISA Detection Kit

»

<
>

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.