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Protein A ELISA Kit

GenScript Protein A ELISA Kit is developed for the accurate quantification of native Protein A, recombinant Protein A variants and MabSelect SuRe™ (Alkali-tolerant recombinant protein A) in the presence or absence of antibodies.
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L00430

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Product Description Protein A affinity chromatography is widely used for antibody purification. Protein A ligand leakage, even though covalently conjugated to the chromatography support, is a big problem for pharmaceutical industry. And it also probably causes false results in immunological assays if antibody product is contaminated with Protein A.
GenScript Protein A ELISA Kit is developed for the accurate quantification of native Protein A, recombinant Protein A variants and MabSelect SuRe™ (Alkali-tolerant recombinant protein A) in the presence or absence of antibodies. The kit offers a sensitive assay for users in pharmaceutical industry to measure mini amount of Protein A in therapeutic antibody products.  Antibody suppliers can use the kit to evaluate Protein A containment level of the products. It also brings convenience to the manufacturers of Protein A affinity chromatography media in the process of monitoring leaching characteristics of the media under specific conditions.
This kit detects residual Protein A in a sandwich immunoassay.  It utilizes two clones of Protein A monoclonal antibodies that bind Protein A at different domains.  One monoclonal antibody is pre-coated on the polystyrene microtiter plate to capture Protein A in samples.  Another monoclonal antibody with Biotin conjugated is then added to the plate to interact with Protein A. Subsequently horseradish peroxidase (HRP) labeled with streptavidin is added to bind the second antibody. The final step involves the addition of TMB Solution. The color intensity is proportional to the amount of Protein A present in the test samples.
Sample process is crucial to the Protein A quantification assay. Leached Protein A can interact with Fc region of antibody, which negatively influence the accurate quantification of Protein A. Protein A ELISA Kit provides an optimized sample treatment protocol, thereby offering more reliable results.
Key Features Accurate quantification of 20 pg/ml Protein A contaminant or 50 pg/ml MabSelect SuRe™
Broad-range detection of natural Protein A, recombinant Protein A variants and MabSelect  SuRe™
Time saving protocol - complete test in 2 hours or less
Ready-to-use ELISA plate with fast & simple sample preparation
Exceptional lot-to-lot consistency guaranteed by high-quality monoclonal antibodies
Wide applications for the quantification of Protein A contaminant and leaching 
Sensitivity Recombinant Protein A:
    LOD: 20 pg/ml    LOQ: 50 pg/ml
MabSelect SuRe™ Protein A:
    LOD: 50 pg/ml
    LOQ: 100 pg/ml
Precision Intra-assay Precision: CV<5%
Inter-assay Precision: CV<10%
Storage The reagents in the kit are stable for 12 months when stored at 2-8 oC.
Kit Contents
Components Quantity Part.No
96-well plate coated with anti-Protein A mAb 1 plate (8 wells x 12 strips) 430-80
Biotin conjugated anti-Protein A mAb 0.5 ml 430-20
Streptavidin-HRP 12 ml 430-30
Recombinant Protein A Standard (10 μg/ml) 1 ml 430-10
MabSelect SuRe™ Protein A Standard (10 μg/ml) 1 ml 430-11
Antibody Dilution Buffer 15 ml 430-90
5x Sample Dilution Solution 30 ml 430-60
20× Wash Solution 40 ml B-A1-70
10% Tween 20 1 ml 430-A0
TMB Solution 12 ml B-A1-40
Stop Solution 6 ml B-A1-50
Plate Sealer 2 pieces NA
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.