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Taq DNA Polymerase without Mg2+

DNA Polymerase is a thermostable DNA polymerase isolated from an E. coli strain that carries the Taq DNA polymerase gene. Taq DNA polymerase is the most common polymerase used for PCR.
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Description

DNA Polymerase is a thermostable DNA polymerase isolated from an E. coli strain that carries the Taq DNA polymerase gene. Taq DNA polymerase is the most common polymerase used for PCR.

Note

Formulation
GenScript Taq DNA Polymerase has been formulated using GenScript's proprietary technology. The enzyme can be shipped at room temperature or even 37°C for seven days without any loss of activity.

Unit Definition
One unit is the amount of enzyme that can incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C.

10 X Reaction Buffer (without Mg2+)
500 mM KCl, 100 mM Tris HCl (pH 9.0 at 25°C), 1% Triton X-100 Buffer. This buffer is optimized for use with 200 µM dNTPs.
Note: If the reaction is performed without this buffer, then add 0.1% Triton X-100 (final concentration) to ensure high activity.
Storage Buffer and Concentration
Taq is delivered in 5 units/µl in 20 mM Tris HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 0.1% Triton X-100 and 50% glycerol.

Storage:
-20°C

*The PCR process is covered by US. Patents numbers 4683195 and 4683202, issued to Cetus and owned by Hoffman-La Roche Inc. GenScript does not encourage or support the unauthorized use of the PCR process. Use of this product is recommended for persons who either have a license to perform PCR or are not required to obtain a license. Sale of this product is restricted to regions or countries where native Taq DNA polymerase patents have been invalidated.

Key Features
  • Terminal transferase activity: Taq DNA Polymerase has terminal transferase activity, which results in the addition of a single nucleotide (adenosine) at the 3' end of the extension product.
  • High purity: No contamination activity has been detected in standard test reactions.
QC
QC Tests
PCR* performance, activity, nuclease

Lane Taq Unit used
1Leading
Brand A		0.1
2 0.25
3 0.5
4Leading
Brand B		0.1
5 0.25
6 0.5
7GenScript0.1
8 0.25
9 0.5


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  • PCR*
  • 3' A-tailing of blunt ends
  • Primer extension
  • DNA sequencing

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