CD70, also named CD27 ligand (CD27L), is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF) and has been designated TNFSF7. CD70 is a cytokine that binds to CD27. Plays a role in T-cell activation. Induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells.
Measured by its binding ability in a functional ELISA.Test result was comparable to standard batch.
Expression System
HEK293
Theoretical Molecular Weight
43.41 kDa
Apparent Molecular Weight
Due to glycosylation, the protein migrates to 55-60 kDa based on Bis-Tris PAGE result.
Formulation
Lyophilized from 0.22μm filtered solution in PBS (pH 6.2).
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability
Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.
Immobilized Mouse CD27, mFc Tag at 2 μg/ml (100 μL/well) on the plate. Dose response curve for CD27 Ligand/CD70 hFc Chimera, Mouse, hFc Tag with the EC50 of 20.4 ng/ml determined by ELISA. »
The purity of CD27 Ligand/CD70 hFc Chimera, Mouse is greater than 95% as determined by SEC-HPLC. »
CD27 Ligand/CD70 hFc Chimera, Mouse on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
CD70, also named CD27 ligand (CD27L), is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF) and has been designated TNFSF7. CD70 is a cytokine that binds to CD27. Plays a role in T-cell activation. Induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells.
