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GenCircle dsDNA_PiggyBac_CMV_Luciferase_GFP

A sequence encoding luciferase_T2A_GFP expression cassette with Piggybac ITRs, transcriptionally promoted by an independent CMV promoter.
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KIOK26

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Description A sequence encoding luciferase_T2A_GFP expression cassette with Piggybac ITRs, transcriptionally promoted by an independent CMV promoter.
Storage Condition Stable for at least 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Quality Specifications
Item Assay Specifications
Concentration Spectrometry A260 Guaranteed quantity
Identity Sanger Sequencing Correct sequence
Identity Restriction Analysis DNA bands of expected sizes detected by agarose gel electrophoresis (AGE)
Purity Spectrometry A260/280 1.8-2.0
Plasmid Topology Agarose Gel Electrophoresis ≥ 90% supercoil
Bioburden Innoculation Test No bacteria/ fungi growth after 72 hours
Endotoxin Gel Clot Test ≤0.01EU/μg
Residual Host RNA Agarose Gel Electrophoresis No bands detected
Residual Host DNA AGE Gray Scale Analysis (GIS) Non-detectable (GIS=0)
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