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GenCircle dsDNA_TRAC_CD19_GFP

A sequence encoding CD19CAR_T2A_GFP (GFP as reporter) and 300bp of TRAC upstream/down stream homology arms. The transcription is promoted by endogenous TRAC promoter of T cell.
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Description A sequence encoding CD19CAR_T2A_GFP (GFP as reporter) and 300bp of TRAC upstream/down stream homology arms. The transcription is promoted by endogenous TRAC promoter of T cell.
Storage Condition Stable for at least 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Quality Specifications
Item Assay Specifications
Concentration Spectrometry A260 Guaranteed quantity
Identity Sanger Sequencing Correct sequence
Identity Restriction Analysis DNA bands of expected sizes detected by agarose gel electrophoresis (AGE)
Purity Spectrometry A260/280 1.8-2.0
Plasmid Topology Agarose Gel Electrophoresis ≥ 90% supercoil
Bioburden Innoculation Test No bacteria/ fungi growth after 72 hours
Endotoxin Gel Clot Test ≤0.01EU/μg
Residual Host RNA Agarose Gel Electrophoresis No bands detected
Residual Host DNA AGE Gray Scale Analysis (GIS) Non-detectable (GIS=0)
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